Effect of TMPD or ascorbate (ASC) in combination with MTX on cell viability.

<p>Cells (6×10⁴) were incubated in 1 ml of medium with TMPD (A) or ASC (B) either alone or in combination with MTX at the indicated concentrations, for 3 or 6 days, respectively, and cell viability was determined using the MTT assay. TMPD or ASC were added to the cells 6 h before MTX. Results are expressed as the percentage of surviving cells compared to the control (untreated cells) and represent the mean ± SE of 3 experiments. * <i>p</i><0.05.