Effect of veratridine (VERA) and GSH on MTX induced apoptosis.

<p>Cells were incubated with veratridine alone or in combination either with MTX or MTX plus GSH, at the indicated concentrations. Veratridine was added 6 h before MTX. Incubation with exogenous GSH started 8 h before the addition of MTX. In the triple combination, cells were incubated 2 h with GSH, then veratridine was added and 6 h later, treatment with MTX was performed. Apoptosis was assessed 18 h after MTX addition by changes in mitochondrial membrane potential as determined by the Rhodamine 123/Propidium Iodide assay and it is expressed in fold change compared to untreated cells. Results represent the mean ± SE of 3 different experiments. Staurosporine (STP) was used as a positive control. * <i>p</i><0.05.