Application of bar-code to cell counting studies.

<p><b>A,</b> Concept of bar-code for mixing differentially marked FP_NLSFP expressing cells. <b>B,</b> Differential response of two LNCaP-C4-2 cell subclones to an inhibitor of cell growth (actinomycin D). <b>C,</b> em1/em2 ratios of all cells within a representative well (x-axis) compared to the intensities of each cell in the em1 channel. <b>D,</b> LNCaP-C4-2 cells mixed, co-plated, treated exactly as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0063286#pone-0063286-g005" target="_blank">figure 5B</a> then separated according to the bar-code showed similar treatment responses to the individually plated cells. Growth measurements are shown as the mean +/− sd from 8 (Fig. 5B) or 16 (Fig. 5D) wells for each treatment condition. *, statistically significant (p<0.01) increases or decrease in cell number relative to vehicle-treated cells; #, statistically significant (p<0.01) increase in cell number of DHT/actinomycin D treated wells relative to actinomycin D-treated wells.