CD200RLc and CD200RLe can generate activating signals when triggered by specific mAb.

<p>(A) Flow cytometry plots showing expression of CD200RLc (left panel) and CD200RLe (right panel) (solid tinted lines) compared to the control mAb (dashed lines) on RBL.2H3 cells stably transduced with either CD200RLc or CD200RLe together with mouse DAP12. (B) RBL.2H3 cells, transduced with CD200RLc and DAP12, CD200RLe and DAP12 or mock vectors, were plated and soluble OX110, OX131, OX132 or no mAb were tested by overnight stimulation. The level of degranulation was measured by assaying β-hexosaminidase in the cell lysate and supernatants. Antibody stimulated groups were compared with no antibody control group with statistically significant results indicated by *** (p<0.001; representative of three experiments).