hCD4/R5/cT1 mouse CD4+ T cells and CD11b+ myeloid-lineage cells are susceptible to HIV-1 infection.

<p>(<b>A</b>) Anti-CD3/CD28-stimulated hCD4/R5/CT1 mouse CD4 T lymphocytes and GM-CSF-stimulated hCD4/R5/cT1 mouse bone marrow-derived myeloid-lineage cells were plated in 48 well plates (1×10⁵ cells/well) and inoculated with NLEng1i-CH077.ecto or NLEng1i-Bal.ecto. After 3 days of culture, HIV-1-infected CD4+T cells (upper panels) and CD11c+ myeloid-lineage cells (lower panels) were quantified by flow cytometry based on their expression of GFP. (<b>B</b>) Anti-CD3/CD28-stimulated hCD4/R5/CT1 mouse and human CD4 T lymphocytes and GM-CSF-stimulated hCD4/R5/cT1 mouse bone marrow-derived myeloid lineage-committed cells and human monocytes were infected with NL-LucR.T2A-BaL.ecto. The cells from triplicate or duplicate cultures were harvested on day 3, 5 and 7 and the average LucR activity in the cell lysates ± STE is shown. (<b>C</b>) GM-CSF-stimulated hCD4/R5/cT1 myeloid lineage-committed cells were either untreated or treated with indinivir and infected with NL-LucR T2A-BaL.ecto. The cells from duplicate cultures were harvested on day 3, 5 and 7 and the average LucR activity in the cell lysates ± STE is shown.