In vivo HIV-1 infection of hCD4/R5/cT1 mice. (A) hCD4/R5/cT1 mice and wild-type mice were intrasplenicly injected with NL4-LucR.T2A-Bal.ecto.

<p>LucR activity in the splenic lysates from the mice was measured at 1 week, 2 weeks and 4 weeks (n = 5–8 mice/group) after infection. For all experiments, the average LucR activity in the spleens of the mice in each group ± STE is shown. (B) hCD4/R5/cT1 mice (n = 2) or a control C57BL/6 mouse were intrasplenicly injected with NL4-LucR.T2A-Bal.ecto and 7 days later the spleens were harvested. After ex vivo injection with RediJect Coelenterazine h, bioluminescent and grey-scale images of isolated spleens from the infected hCD4/R5/cT1 mouse spleens (left and center) and the control C57BL/6 mouse (right) were captured with the IVS Spectrum imager and bioluminescence intensity represented in a pseudocolor image indicating photon counts/second are shown. (C) HIV-1 RNA levels in the plasma of hCD4/R5/cT1 and wild-type mice (n = 3 mice/group) were quantified at 2 and 3 weeks after intrasplenic injection with NL4-LucR.T2A-Bal.ecto. (D) Five days after hCD4/R5/CT1 mice or wild-type mice were intravaginally challenged with NL4-LucR.T2A-Bal.ecto (n = 5 mice/group), the LucR levels in leukocytes isolated from the vaginal tissues were determined.