HIV-1 infection of hCD4/R5/cT1 mice is inhibited by VRC01 antibody secreted in vivo by transduced B cells.

<p>hCD4/R5/cT1 mice were uninjected (n = 4 mice) or intrasplenicly injected with highly purified primary B cells which were either untransduced (n = 4 mice) or transduced with the VRC01-expressing lentivirus (n = 4 mice). Two days later, the mice were all inoculated intravenously with NL-LucR-T2A-Bal.ecto. Seven days later, the mice were sacrificed. (<b>A</b>) The level of VRC01 antibody in the serum was determined by an ELISA assay (<b>B</b>) The LucR activity in the mouse spleens was quantified. The average VRC01 serum antibody levels and LucR activity in the spleens for the mice in each group +/− STE is shown.