<i>In situ</i> PRS mapping of the collagen orientation in −45 and 45 degrees tilted dried RTT.

<p>(A) and (C) show maps obtained by fitting thirteen Raman images collected with different polarization angles of the incident laser light. The direction of arrows indicates the orientation of collagen molecules, their length represents the amplitude of the fitting curve, and the color code is the average intensity of the amide I band. (B) and (D) are example of experimental points extracted from the area marked in (1) and (2), respectively.