<p>The amplified product should contain the ATG. The blunt-end DNA segment can be cloned in all the three vectors.</p
<p>The vector contains two appropriately oriented BsaI sites (A) upon digestion with BsaI linearized...
<p>(<b>A</b>) PCR products amplified to identify unknown sequences flanking a region of known sequen...
<p>(A) Linear DNA displayed as a ribbon with six genome regions labelled in three different colors. ...
<p>(A) A diagram of single fragment cloning in a binary base vector. Base vector containing a <i>lac...
(a) DNA model depicted in ribbon diagram (b) AT and (c) GC base pair drawn in stick model.</p
<p>QC cloning vectors can be prepared by amplification of a DNA fragment containing a visible select...
<p>Overlapping PCR method is shown with only the strands that will be extended by the DNA polymerase...
<p>Individual exons are amplified in the first PCR (exon PCR) following standard PCR protocol with s...
<p>(A) Top: Site-directed mutagenesis using oligonucleotide is shown using K103N as an example. Desi...
<p>General cloning procedure from PCR fragments to pBRT7Qβ with transient cloning in the pUC18-casse...
<p>The symbols for Pfu DNA polymerase, ApeUDG, dU-carrying stem-loop oligonucleotide, and dU nucleot...
<p>Restriction sites within the MCS were unique digestion sites in the vector. The structure of pCMF...
<p>Single strands of DNA are represented by orange and black lines, and arrowheads indicate 3′ ends....
(A) Schematic diagram illustrating how vector pLF5CsfGFP was generated. Fragments 1 and 2 were ampli...
<p>(A) Design for PCR detection of recombination products. The diagrams show the hypothetical scenar...
<p>The vector contains two appropriately oriented BsaI sites (A) upon digestion with BsaI linearized...
<p>(<b>A</b>) PCR products amplified to identify unknown sequences flanking a region of known sequen...
<p>(A) Linear DNA displayed as a ribbon with six genome regions labelled in three different colors. ...
<p>(A) A diagram of single fragment cloning in a binary base vector. Base vector containing a <i>lac...
(a) DNA model depicted in ribbon diagram (b) AT and (c) GC base pair drawn in stick model.</p
<p>QC cloning vectors can be prepared by amplification of a DNA fragment containing a visible select...
<p>Overlapping PCR method is shown with only the strands that will be extended by the DNA polymerase...
<p>Individual exons are amplified in the first PCR (exon PCR) following standard PCR protocol with s...
<p>(A) Top: Site-directed mutagenesis using oligonucleotide is shown using K103N as an example. Desi...
<p>General cloning procedure from PCR fragments to pBRT7Qβ with transient cloning in the pUC18-casse...
<p>The symbols for Pfu DNA polymerase, ApeUDG, dU-carrying stem-loop oligonucleotide, and dU nucleot...
<p>Restriction sites within the MCS were unique digestion sites in the vector. The structure of pCMF...
<p>Single strands of DNA are represented by orange and black lines, and arrowheads indicate 3′ ends....
(A) Schematic diagram illustrating how vector pLF5CsfGFP was generated. Fragments 1 and 2 were ampli...
<p>(A) Design for PCR detection of recombination products. The diagrams show the hypothetical scenar...
<p>The vector contains two appropriately oriented BsaI sites (A) upon digestion with BsaI linearized...
<p>(<b>A</b>) PCR products amplified to identify unknown sequences flanking a region of known sequen...
<p>(A) Linear DNA displayed as a ribbon with six genome regions labelled in three different colors. ...
DOI
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