Expression of <i>ALS</i>, <i>ACCase</i> and five <i>CYP</i> genes in individual <i>Lolium</i> sp. plants in four groups of samples.

<p>A group of samples consists of the plants from the same population that have been used to assess the effect of the same herbicide in sampling IM2 or P2 (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0063576#pone-0063576-t002" target="_blank">Table 2</a>). For every individual plant, the relative expression levels of each gene measured in the absence of herbicide (BT) and 24 hours after herbicide application (24HAT) are connected by a solid line for herbicide-resistant plants, a dotted line for herbicide-sensitive plants or a dashed-and-dotted line for plants with a moderately resistant phenotype. A given colour in all panels in a column (i.e., in a given group of samples) indicates the same individual plant. Panels on lanes A to G, relative expression levels for <i>ALS</i>, <i>ACCase</i>, <i>CYP71R4</i>, <i>CYP72A</i>, <i>CYP81B1</i>, <i>CYP81A</i> and <i>CYP92A</i>, respectively. The herbicide considered is indicated below the population code using the sampling code (IM2, iodosulfuron+mesosulfuron; P2, pyroxsulam, see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0063576#pone-0063576-t002" target="_blank">Table 2</a>). Identical letters in a given panel and in a given experimental modality (BT or 24HAT) indicate relative expression levels that are not significantly different.