Clathrin and AP2 likely act in the same genetic pathway as CED-1 and CED-6.

<p>(A and B) Quantification of germ cell corpses in <i>ced-1(e1735)</i> and <i>ced-6(n2095)</i> (A) or <i>ced-2(n1994)</i> and <i>ced-5(n1812)</i> (B) mutants treated with Ctrl RNAi and RNAi of <i>chc-1</i>, <i>dpy-23</i> and <i>apb-1</i>. Cell corpses were scored in animals at 24 and 36 h after the L4 molt. Error bars represent SEM. Comparisons were made between control RNAi and RNAi of <i>chc-1</i>, <i>dpy-23</i> and <i>apb-1</i> using unpaired <i>t</i>-tests. ** <i>p</i><0.001; all other points had <i>p</i>>0.05. (C) Representative images of cell corpse labeling by APA-2::GFP in N2, <i>ced-1(e1735)</i>, <i>chc-1(RNAi)</i> and <i>ced-6(RNAi</i>) germ lines. (D) Representative images of cell corpse labeling by mCherry::CHC-1 in N2, <i>apb-1(RNAi), ced-1(RNAi)</i> and <i>ced-6(RNAi)</i> germ lines. In (C) and (D) arrows indicate cell corpses labeled by APA-2::GFP or mCherry::CHC-1 while arrowheads indicate unstained corpses. Bars, 10 µm.