Western Blot analysis of p38 phosphorylation in dependence of treatment with TGF-β and/or 1α,25(OH)₂D₃.

<p>(A) Western Blot analysis of p38 protein and phosphorylated p38 protein in THP-1 incubated for 24 h with 1 ng/ml TGF-β (T), 50 nM 1α,25(OH)₂D₃ (V), both agents (TV) or without treatment (w/o). The relative phospho-p38 level (normalized to total p38 protein) of TGF-β/1α,25(OH)₂D₃ treated cells was set to 1. (B) THP-1 cells were treated with (TV) or without (w/o) 1 ng/ml TGF-β and 50 nM 1α,25(OH)₂D₃ for 24 h after 30 min pre-incubation with or without the indicated inhibitors. Applied concentrations of oxozeaenol and zeaenol were 0.3 µM, respectively. Phosphorylated p38 compared to whole p38 protein was analyzed by western blot. Relative protein amount of TGF-β/1α,25(OH)₂D₃ treated cells was set to 1. Statistical analysis of (A) and (B) was done with one sample t-test (*P<0.05; **P<0.01).