Effects of C5 activation inhibitor OmCI during IAV infection on leukocyte transmigration and protein leakage.

<p>C57BL/6J mice were infected intranasally with influenza or Mock infected as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0064443#pone-0064443-g001" target="_blank">Figure 1</a>. OmCI treated mice received the protein prior to IAV infection and daily thereafter, while vehicle group received PBS, until one day before the indicated time point. At the 1, 3 and 6 days after infection, mice were euthanized and BAL performed. A) Total number of leukocytes, B) number of neutrophils and C) number of macrophages, D) number of lymphocytes recovered from the airways; E) total protein quantification in BALF. Data are presented as Mean ± SEM. *, ** and *** for p<0.05, p<0.01 and p<0.001 respectively, when compared to Mock group; # and ## for p<0.05, p<0.01 respectively, when compared to Vehicle group sampled on the same day (One-way ANOVA, Newman-Keuls Multiple Comparison test).