High-content cell cycle analysis.

<p>HT29 cells treated for 48 hours with the indicated treatments in a 384-well plate were fixed and stained with Hoechst 33452, imaged, and integrated staining intensity of individual nuclei was estimated as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0063583#s2" target="_blank">Materials and Methods</a>. <b>A</b>. DNA content histograms for log-2 transformed DNA intensity values normalized to the modal value of the 2N DNA. Automatic classification into sub-G1, 2N, S-phase, 4N and 8N populations is indicated. <b>B</b>. DNA content histograms acquired by flow cytometry, raw FL2-A data was normalized and binned in the same way as high-content data. <b>C</b>. Quantitation of the sub-population fractions of the histograms.