Cell cycle profiles derived from high-content assay.

<p>Cells in the same images used for direct cell counting were classified into five cell cycle bins by integrated DNA intensity. <b>A.</b> Stacked bars show the relative frequencies of the sub-populations at the indicated concentrations. Each bar is the average of two wells. Black circles indicate relative cell number. <b>B.</b> Histograms of integrated DNA intensity derived from the images from individual wells showing the change in cell cycle profile from EC₉₀ (upper panels) to higher doses of etoposide, gemcitabine and VX-680.