Effects of drug treatment on mitochondrial function.

<p>Basal oxygen consumption rate (OCR) determined for cells treated with the indicated compounds (etoposide, 10 µM; gemcitabine 0.1 µM; paclitaxel 0.01 µM; PD901 1 µM, VX-680 0.2 µM) were normalized for cell number. Per-cell OCR is compared with normalized ATP-generated RLU (<b>A</b>) and mitochondrial mass (<b>B</b>). Cells analyzed for mitochondrial mass by MitoTracker Deep Red staining were also stained with the mitochondrial membrane potential-sensitive dye TMRE, and the mean integrated intensities compared (<b>C</b>). All data were normalized as a ratio of the mean DMSO-treated values and are the mean of four replicate wells, error bars show standard deviation.