Variable foliation patterns among <i>hGFAP-Cre, β-catenin^fl/fl</i> mice.

<p>The white dotted line indicates the position of the pr, or the deepest fissure in the anterior lobe (pcu in the control mice and dp in the mutant mice). In the mutant mice recognizable fissures were designated by an asterisk after the corresponding name. Other fissures were assigned from rostral to caudal as ‘a1, a2...an’. (A) Example medial and lateral sections of four P4 littermates stained for calbindin and counterstained with DAPI. While the foliation patterns were similar in the two control cerebella, they were different in the two mutant cerebella. In both mutant 1# and mutant 2#, one ectopic fissure appeared between the two dotted lines (a4 and a5, respectively). Therefore the shape of Lobule IV/V was changed. From anterior to deepest fissure, there is only one fissure (pct) in the control mice, but there were two or more fissures in the mutant mice (a1 and a2 in mutant 1#; a1, a2 and a3 in mutant 2#). Lateral sections showed simpler folia than medial sections. Note that the mutant egl tended to be torn off (arrowhead). (B) Example medial sections of one control and three mutant cerebella at P7 or P8 stained with antibodies to calbindin and NeuN. In mutant 1# and mutant 2# one ectopic fissure (a4) appeared between the two dotted lines. There were more fissures anterior to dp in all the three mutant cerebella (a1 and a2 in mutant 1# and 2#; a1, a2 and a3 in mutant 3#). Arrows indicate the deeply-located lobules. Rostral is to the left and dorsal is to the top in each photograph. Abbreviations: itc, intercrural; pct, precentral; pcu, preculminate; pl, posterolateral; ppy, prepyramidal; pr, primary; sec, secondary fissure. Scale bars: 250 μm.