Effect of <i>nlgst1-1</i> dsRNA on the mortality of BPH nymphs.

<p><i>nlgst1-1</i> dsRNA that targets the knockdown of <i>nlgst1-1</i> in BPHs was dissolved in artificial diets at the indicated concentrations (0, 0.2, 0.4 and 0.8 µg/ml), BPH nymphs were then fed on these liquid diets held in the two layers of Parafilm M in controlled growth chambers (90% r.h., 28°C, 14 hr photoperiod) for 96 hrs. Fresh diets containing the indicated concentrations of <i>nlgst1-1</i> dsRNA were changed every day. The diet containing 0.8 µg/ml <i>GFP</i>-dsRNA was used as control (0 µg/ml <i>nlgst1-1</i> dsRNA). The average mortalities of BPHs with the increase of <i>nlgst1-1</i> dsRNA concentrations were plotted at 24, 48, 72 and 96 h.p.f. Data represent the means ± SD from three independent experiments with triplicate groups examined for each treatment in each experiment.