Effects of BDNF treatment on pCREB in the cortex and striatum of R6/2 mice.

<p>Representative confocal laser scanning microscopy images of dual-label immunofluorescence for Neun (red) and pCREB (green) or CALB (red) and pCREB in the cortex (A–C) or striatum (D–F) of wild-type, (A–D) vehicle R6/2 mice (B–E), or (C–F) R6/2 mice treated with BDNF at 13 weeks of age. (G–H) Quantification of the intensity of pCREB immunoreactivity associated with Neun-labeled cortical neurons (G) or CALB-labeled striatal neurons. (I–J) pCREB levels were analyzed by Western blotting of protein extracts obtained from the striatum and cortex of WT and R6/2 mice treated with vehicle or BDNF at 12 weeks of age.