Expression of maturation markers in human dendritic cells according to extracellular Ca²⁺ concentration.

<p>DC were cultured with increasing concentrations of Ca²⁺<b>(in mM)</b>. Cells were harvested and double or quadruple staining was assessed by FACS analysis for the 4 conditions. <b>A</b> – <i>CD83 expression is increased by extracellular Ca2+ concentration</i>. The population showed on this panel is gated on DC-Sign labeling. The percentage of double (CD86 and CD83) positive cells is shown in panel A. Results are representative of 7 independent experiments. <b>B</b> – <i>Expression of maturation markers in relation to extracellular Ca²⁺ concentrations</i>. The same population expressed CD80, CD86, HLA-DR and CD83 in increasing proportions in increasing extracellular Ca²⁺ concentration. The percentage of quadruple positive cells is shown in panel B. Graph bars represent the mean of 6 independent experiments (mean ± SD, *p<0,05). <b>C</b> – <i>CD25 expression in relation to extracellular Ca²⁺</i>. Grey histograms represent the expression of the cell-surface marker CD25. Results represent one out of 7 independent experiments.