Bioactive compounds of <i>Rhynchosia viscosa</i> in the vascular outgrowth assay.

<p>IC₅₀ curves and values were determined for each of the bioactive constituents of the methanolic extract of <i>R. viscosa</i>. Each compound, at six different concentrations, was assessed for their effect in the inhibition of intersegmental vessel (ISV) growth. <b>A</b> to <b>F</b>, all embryos are 48 hours post-fertilization (hpf), with anterior to the left, scale bar = 10 µm. <b>A</b>, untreated control (DMSO 1%); <b>B</b>, zoom of <b>A</b> (dashed box) showing normal outgrowth of intersegmental vessels (ISV) along the trunk of the larva (arrows); <b>C</b>, embryo treated with 50 µM genistein; <b>D</b>, embryo treated with 100 µM rhynchoviscin; <b>E</b>, embryo treated with 50 µM licoisoflavone A; <b>F</b>, embryo treated with 50 µM sophoraisoflavone. Arrowheads point the interconnection zone between the dorsal aorta and the posterior cardinal vein, arrows point the ISV; <b>G</b>, IC₅₀ curves and values (µM) for each of the bioactive compounds of <i>R. viscosa</i>.