Preferential engagement of FcγRI results in uptake of neutralized DENV immune complexes.

<p>(A) Sorting of AF488-DENV infected cells using fluorescence activated cell sorter at 120 mins post infection after synchronization, in absence of antibody (virus only), with neutralizing (3.125 µg/mL) or sub-neutralizing (0.39 µg/mL) antibody concentrations. For mock infection, cells were exposed to h3H5 antibody only. Percentages of AF488-DENV positive cells (green histogram) are numerically indicated. (B) Cellular localization of AF488-DENV immune complexes at neutralizing or sub-neutralizing concentration of h3H5 after 120 mins post infection. LAMP-1 is in red. DENV is in green and FcγRI/FcγRII is in blue. White areas in the merged image indicate the presence of co-localization. Scale bar is 10 µm. (C) Percent co-localization of AF488-DENV opsonized with either neutralizing or sub-neutralizing levels of h3H5, with respect to FcγRI or FcγRII at 120 mins post infection using the confocal microscope, Zen 2009 Software. Images shown are representative of at least 2 separate experiments. Data are represented as mean ± s.e.m. * p<0.01.