Breast tumor cells expressing high or low levels of HA adhere differently to cells during low flow conditions.

<p>(A) The number of HS-578T and MCF-7 cells adhered to COS-7^LYVE-1(+) and COS-7^LYVE-1(−) cells under 0.1 dyn/cm² low shear stress was evaluated by counting 5 random views of fluorescence microscope images. Adhered HS-578T and MCF-7 cells appear as blue spheres (a–d). (B) Quantitative evaluations of the data representing HS-578T and MCF-7 cell adhesion on the monolayer of COS-7 cells are shown, and each assay was performed in triplicate. The results are shown as the mean ± SD of triplicate wells from a typical experiment and are expressed as a fold increase relative to control pEGFP-N1 vector transfected COS-7 cells. *P<0.05 versus the indicated transfected pEGFP-N1 vector control COS-7 cells. (C) The number of HS-578T and MCF-7 cells adhered to SVEC4-10 cells before and after blocking with LYVE-1 antibody, isotype antibody under 0.1 dyn/cm² low shear stress was evaluated by counting 5 random views of fluorescence microscope images (g–l). (D) Quantitative evaluations of the data from HS-578T and MCF-7 cell adhesion on the monolayer of SVEC4-10 cells are shown. Each assay was performed in triplicate. The results are shown as the mean ± SD of triplicate wells from a typical experiment and are expressed as a fold decrease relative to SVEC4-10 cells without treatment. *P<0.05 versus SVEC4-10 cells without treatment.