Differential effects of renal tubular epithelial cells on T-cell migration.

<p>(A) PBMCs were activated with anti-CD3/CD28, IL-2 and IFN-γ and placed in a 3.0 µm pore transwell system upon unstimulated TECs or 24 hours IFN-γ/TNF-α stimulated TECs. Activated CD4⁺ T cells expressed 76% CXCR3 and 68% CCR6. (B) Significant migration of CD4+CXCR3+ T cells, containing Th1 cell pool, by activated TECs was observed (P<0.05). No migration of CD4+CCR6, containing Th17 cell pool, could be detected. CD7⁺ and CD16^-/CD56^- lymphocytes were discriminated as anti-CD3/CD28 activated T lymphocytes. (C and D) Transwell migration results of 9 individual experiments using unstimulated TECs vs. stimulated TECs are shown. The total cell number of migrated CD4⁺CXCR3⁺ and CD4⁺CCR6⁺ T cells are depicted on the Y –axis. Results were obtained by flow cytometry using fixed time point and fixed volume. * Significant increase (P <0.05).