ELISA to determine relative anti-rK39 titers in serum sample.

<p>Serum samples were diluted at 1∶6400 dilution. The 2 circled samples in blood-negative, serum-positive samples were re-evaluated and found to be positive of the rK39 immunochromatographic RDT. Compared to Blood (−)/Serum (−), the difference with Blood (+)/Serum (+) was p = 2.3×10⁻²⁹; the difference with Blood (−)/Serum (+) was p = 0.017; the difference Blood (+)/Seum (−) was p = 0.31.