<p>(A) Experimental design: Donor (B6.SJL) mice and recipient (C57BL/6 and CD40L-deficient) mice were concurrently primed with 10<sup>7</sup> cfu Lm-QV. 21 days later, CD8+ T cells were purified from B6.SJL spleens and transferred into B6 and B6.<i>Cd40L</i>-/- mice. 24 h post-transfer, recipients were boosted with 5×10<sup>6</sup> cfu Lm-QV in the presence of αCD40L or control antibody. OVA<sub>257–264</sub>-specific memory CD8+ T cell responses were assessed 5 days following boost using intracellular cytokine staining for IFN-γ (<i>n</i> = 5). (B) Total OVA<sub>257–264</sub>-specific (IFN-γ+) CD45.1+CD8+ T cells per animal (mean, ±SEM). *, P<0.05, **, P<0.01, Mann-Whitney. (C) Fold expansion of donor CD45.1+ IFN-γ + OVA<sub>257–264</sub>-...
<p>Three different T cell subsets were used for adoptive transfer 4 weeks post infection: CD4<sup>+<...
The interaction between CD40 and its ligand (CD154) plays a critical role in the activation of the i...
<p>Mice were primed with PBS or anti-DEC:OVA+anti-CD40, and boosted with anti-DEC:OVA+anti-CD40 at d...
<p>Mice were primed with 10<sup>7</sup> cfu Lm-QV or 10<sup>6</sup> pfu VV-OVA and boosted 21 days l...
<p>Mice primed with Lm-OVA were boosted 21 days later with the indicated dose of Lm-OVA. 5 days post...
<p>Mice were immunized with 10<sup>6</sup> pfu VV-OVA and boosted 21 days later with 5×10<sup>6</sup...
<p>(A–B) Mice were primed with 10<sup>7</sup> cfu Lm-OVA, 10<sup>6</sup> pfu VV-OVA, or HBSS and boo...
<p>A. CD40-B cell vaccination generates CD8<sup>+</sup> Te cells but not Tm cells. 10<sup>6</sup> fe...
The ability of memory CD8+ T cells to rapidly proliferate and acquire cytolytic activity is critical...
<p>Lymphopenic RAG<sup>−/−</sup> mice reconstituted with CellTrace Violet-labeled LN cells from cong...
<p>Thy1.2 C57BL/6 mice that healed their primary low dose (1×10<sup>3</sup>) or high dose (2×10<sup>...
<p>(A) WT, T-bet−/− (TKO), Eomes−/− (EKO), or T-bet/Eomes DKO pmel-1 T cells were cultured in Th1 co...
<p>(A) <i>Hdac1<sup>f/f</sup></i> and <i>Hdac1<sup>f/f</sup>Cd4Cre</i> P14 CD8<sup>+</sup> T cells (...
<p>B6-Ly5.2/Cr mice were transplanted with BALB/c skin grafts and were treated with 10<sup>7</sup> B...
During homeostatic expansion, peripheral T cells proliferate in response to lymphopenia, in the abse...
<p>Three different T cell subsets were used for adoptive transfer 4 weeks post infection: CD4<sup>+<...
The interaction between CD40 and its ligand (CD154) plays a critical role in the activation of the i...
<p>Mice were primed with PBS or anti-DEC:OVA+anti-CD40, and boosted with anti-DEC:OVA+anti-CD40 at d...
<p>Mice were primed with 10<sup>7</sup> cfu Lm-QV or 10<sup>6</sup> pfu VV-OVA and boosted 21 days l...
<p>Mice primed with Lm-OVA were boosted 21 days later with the indicated dose of Lm-OVA. 5 days post...
<p>Mice were immunized with 10<sup>6</sup> pfu VV-OVA and boosted 21 days later with 5×10<sup>6</sup...
<p>(A–B) Mice were primed with 10<sup>7</sup> cfu Lm-OVA, 10<sup>6</sup> pfu VV-OVA, or HBSS and boo...
<p>A. CD40-B cell vaccination generates CD8<sup>+</sup> Te cells but not Tm cells. 10<sup>6</sup> fe...
The ability of memory CD8+ T cells to rapidly proliferate and acquire cytolytic activity is critical...
<p>Lymphopenic RAG<sup>−/−</sup> mice reconstituted with CellTrace Violet-labeled LN cells from cong...
<p>Thy1.2 C57BL/6 mice that healed their primary low dose (1×10<sup>3</sup>) or high dose (2×10<sup>...
<p>(A) WT, T-bet−/− (TKO), Eomes−/− (EKO), or T-bet/Eomes DKO pmel-1 T cells were cultured in Th1 co...
<p>(A) <i>Hdac1<sup>f/f</sup></i> and <i>Hdac1<sup>f/f</sup>Cd4Cre</i> P14 CD8<sup>+</sup> T cells (...
<p>B6-Ly5.2/Cr mice were transplanted with BALB/c skin grafts and were treated with 10<sup>7</sup> B...
During homeostatic expansion, peripheral T cells proliferate in response to lymphopenia, in the abse...
<p>Three different T cell subsets were used for adoptive transfer 4 weeks post infection: CD4<sup>+<...
The interaction between CD40 and its ligand (CD154) plays a critical role in the activation of the i...
<p>Mice were primed with PBS or anti-DEC:OVA+anti-CD40, and boosted with anti-DEC:OVA+anti-CD40 at d...