Cytosolic γ-catenin is stabilized in response to GSK-3 inhibition/ablation in wild-type mESCs.

<p>(A) Robust cytosolic stabilization of β-catenin and γ-catenin in mESCs after treatment with CHIR99021 (15 µM for ∼24 hours), and in mESCs lacking both isoforms (α and β) of GSK-3 (DKO), as assessed by western blotting of hypotonic lysates. Increases in β-catenin and γ-catenin levels were not clearly observed using whole cell lysates for western blotting purposes after the same treatment. (B) β-catenin and γ-catenin stabilization in mESCs after Wnt pathway activation using Wnt3a-conditioned medium (∼24 hours). (C) β-catenin and γ-catenin stabilization in DKO mESCs is reversed by re-expression of either wild-type GSK-3α or GSK-3β, but not their kinase-dead forms (K148A and K85A, respectively).