HIV Env staining of intact live cells.

<p>HEK293T/17 cells transfected with the indicated VSV-G containing Env were stained with (A) anti-HA, and (B) anti-VSV-G monoclonal antibodies. Only intact live cells were selected for analyses of Env exposure to reference monoclonal antibodies. (A) Total cell surface Env expression was determined by staining cells with anti-HA, targeting HA epitope tag inserted into gp120. (B) CTT exposure was determined by staining cells with anti-VSV-G, targeting the VSV-G epitope tag in the CTT sequence as demonstrated in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0065220#pone-0065220-g001" target="_blank">Figure 1</a>. The line at 20% staining is for visual reference only.