Clonogenic survival in relation to <i>MSH3</i> knockdown in HCT116+3+5 and SW480 cells treated with SN-38, oxaliplatin or 5-FU.

<p>A, D, The effect of suppression of <i>MSH3</i>, using an inducible shRNA system (doxycycline 1 µg/ml, DOX+), upon chemosensitivity of cell lines to treatment with SN-38 (A) or oxaliplatin (D) [48 h] relative to control cells (DOX-). B,C,E,F, HCT116+3+5 and SW480 cells lines with constitutive knockdown of <i>MSH3</i> by shRNA were treated with SN-38 (B, C) or oxaliplatin (E, F) and clonogenic survival was determined relative to cells with control shRNA. G,H,I, HCT116+3+5 cells with <i>MSH3</i> suppression (DOX+) or gene knockdown using shRNA were treated with 5-FU for 48 h and clonogenic survival was determined compared to control cells (DOX- or non targeted shRNA). Data are presented as mean ± standard errors <i>vs</i> control cells from at least 3 independent experiments. Statistical significance was determined by a two-sided Student's t test (* P<0.05; † P<0.01).