ISH analysis of MLF1-IP mRNAS expression in p1, p10 and adult mice.

<p>Sagittal sections of p1, p10 and adult mice were stained with crystal violet (I), antisense probes (II) and sense probes (control) (III) as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0063783#pone-0063783-g001" target="_blank">Figure 1</a>. Column A: sections of a newborn (p1) mouse with MLF1-IP mRNA labelling seen in the retina, cerebellum, submaxillary gland, thymus, liver, intestine and kidney marginal zone. Column B: sections of a p10 mouse displaying various levels of MLF1-IP expression in different organs, including bone marrow within vertebrae and testis. Non-specific hybridization is noted in dorsal aorta (*), aortic root (**) and alveolar bone (***) according to sense probe hybridization. Column C: sections of an adult mouse showing low-level MLF1-IP mRNA in the cerebellum, thymus, spleen and bone marrow. There is no detectable hybridization in the liver and kidney. Non-specific hybridization is seen in the femoral, skull alveolar bones (*), ribs (**) and in the content of the large intestine (***). Original magnifications: (A) 1.5X; (B) 1.3X and (C) 1.0X.