Quantitative analysis of kainic acid-induced changes in mTOR phosphorylation at Ser2448.

<p>(<b><i>A</i></b>) Representative micrographs of Nissl-stained brain sections with an outline of brain regions used for P-mTOR immunoreactivity (IR) semi-Q-IHC analysis. Areas of cell body layers for mean optical density measurements are surrounded with yellow line while areas used for hippocampal and piriform cortex neuropil analysis are contoured with black line. (<b><i>B</i></b>) Quantification of cellular layer and neuropil P-mTOR immunoreactivity in hippocampi and piriform cortex of control animals (<i>n</i> = 9) and animals treated with kainic acid (KA) and evaluated at 2 h (<i>n</i> = 6) and 24 h (<i>n</i> = 6) post KA. Error bars represent the standard deviation. Asterisks indicate significant changes in neuropil P-mTOR IR vs. control, *<i>p</i><0.05, **<i>p</i><0.01; Mann Whitney U-test. (<b><i>C</i></b>) Quantification of ratio of cellular layer P-mTOR IR to neuropil P-mTOR IR. Error bars represent the standard deviation. *<i>p</i><0.05, **<i>p</i><0.01; Mann Whitney U-test.