Effects of Dox and isorhamnetin on mitochondrial apoptotic pathway in vivo and in vitro.

<p>Mitochondrial injuries in rat heart tissues were observed by transmission electron microscopy (A). H9c2 cardiomyocytes stained with JC-1 dye were visualized by fluorescence microscopy (B). Cytochrome c (cyt c), caspase-9/3, and PARP in rat heart tissues and H9c2 cardiomyocytes were determined by western blot analysis (C). Cont, vehicle treatment; Iso, isorhamnetin treatment; Dox, doxorubicin treatment; Dox+Iso, isorhamnetin and doxorubicin co-treatment.