Live monitoring of Tα1 α-tubulin promoter driven GFP in the neural differentiation process.

<p>At day 0, undifferentiated ALS-iPS-1 (A), C57-iPS-12 (B) and E14 (C) did not express GFP. GFP expression appeared at day 2 of the EBs of ALS-iPS-1 (D), C57-iPS-12 (E) and E14 (F). The expression of GFP increased over time (G–I). (J) FACS analysis indicated that 12.4±2.6% of ALS-iPS-1 differentiated cells was GFP positive, 13.2±2.4% of C57-iPS-12 differentiated cells was GFP positive, while 13.1±1.8% of E14 differentiated cells was GFP positive cells, showing no statistical difference in the number of GFP-positive among the three cell lines (P>0.05). Scale bar: 75 µm.