<p>Lane sdAb-4f, 5f, 6f and Sumof, flow though; Lane sdAb-4p, 5p, 6p and Sumop, purified the single-domain antibody proteins with Ni-NTA agarose; M, protein molecular weight standards, the size markers are 85, 50, 35, 25 and 20 kDa from top to bottom (A). Western-blot analysis of products of recombinant fusion single-domain antibody protein (B).</p
The protein samples in different steps of the purification of fusion proteins (αB-AC and αB-BC) were...
<p>Recombinant proteins were expressed in <i>E. coli</i> BL21 (DE3) or SI and purified from the bact...
<p>M: protein molecular weight markers; lane 1: MT separated with 150 mM imidazole; lane 2: MT separ...
<p>The expression of recombinant fusion proteins were analyzed by SDS-PAGE. M, molecular size protei...
Additional file 4: Figure S4. Molecular weight and purity assessment of sdAb. Figure S4. Assessment ...
The fusion proteins were analyzed by reducing SDS-PAGE (gel 12%) (A), and western blot (B). Lane 1 s...
<p><b>(A)</b> SDS-PAGE analysis of purified full length recombinant AtCyp19-3, and deleted versions ...
SDS-PAGE analysis of the purified recombinant proteins used in this study. M = molecular mass marker...
<p>XeHypBA1 (A), XeHypBA2 (B), XeHypAA (C). M, protein molecular weight marker; lane 1, soluble prot...
<p>a) Composition of the bispecific scDb-IgBD and the monovalent scFv-IgBD fusion protein. b) SDS-PA...
<p>Lane M: protein standards; lane 1, 2: SDS–PAGE analysis of the induced whole cellular lysate; lan...
<p>(A). Primary structure of monomer and combodies of the sdAbs used in our study are shown. (B) Siz...
<p>GST and GST-ST1, -ST2 and -ST3 fusions proteins were expressed in <i>E.coli</i>, extracted, and r...
<p>SDS-PAGE analysis of the purified recombinant Xyl3A. Lanes: 1, the purified recombinant Xyl3A aft...
<p>Lane M: protein marker; Lanes 1–6: TolC (approx. 51 kDa), HAPS_0926 (approx. 25 kDa), OppA (appro...
The protein samples in different steps of the purification of fusion proteins (αB-AC and αB-BC) were...
<p>Recombinant proteins were expressed in <i>E. coli</i> BL21 (DE3) or SI and purified from the bact...
<p>M: protein molecular weight markers; lane 1: MT separated with 150 mM imidazole; lane 2: MT separ...
<p>The expression of recombinant fusion proteins were analyzed by SDS-PAGE. M, molecular size protei...
Additional file 4: Figure S4. Molecular weight and purity assessment of sdAb. Figure S4. Assessment ...
The fusion proteins were analyzed by reducing SDS-PAGE (gel 12%) (A), and western blot (B). Lane 1 s...
<p><b>(A)</b> SDS-PAGE analysis of purified full length recombinant AtCyp19-3, and deleted versions ...
SDS-PAGE analysis of the purified recombinant proteins used in this study. M = molecular mass marker...
<p>XeHypBA1 (A), XeHypBA2 (B), XeHypAA (C). M, protein molecular weight marker; lane 1, soluble prot...
<p>a) Composition of the bispecific scDb-IgBD and the monovalent scFv-IgBD fusion protein. b) SDS-PA...
<p>Lane M: protein standards; lane 1, 2: SDS–PAGE analysis of the induced whole cellular lysate; lan...
<p>(A). Primary structure of monomer and combodies of the sdAbs used in our study are shown. (B) Siz...
<p>GST and GST-ST1, -ST2 and -ST3 fusions proteins were expressed in <i>E.coli</i>, extracted, and r...
<p>SDS-PAGE analysis of the purified recombinant Xyl3A. Lanes: 1, the purified recombinant Xyl3A aft...
<p>Lane M: protein marker; Lanes 1–6: TolC (approx. 51 kDa), HAPS_0926 (approx. 25 kDa), OppA (appro...
The protein samples in different steps of the purification of fusion proteins (αB-AC and αB-BC) were...
<p>Recombinant proteins were expressed in <i>E. coli</i> BL21 (DE3) or SI and purified from the bact...
<p>M: protein molecular weight markers; lane 1: MT separated with 150 mM imidazole; lane 2: MT separ...