Comparison of triphosphate nucleotide structures as bound to wild-type and E->G mutated Fic proteins from class I to III.

<p>Stereo views of the ligand structures after superposition of the FIC domains (not shown). Also shown is the inhibitory glutamate of the wild-type structures. A, ATP as bound to VbhA/VbhT wild-type (white) and the E24G mutant (dark green). B, ATP and AMPPNP as bound to SoFic wild-type (green) and the E73G mutant (orange), respectively. C, AMPPNP as bound to NmFic (blue) and the E186G mutant (pink). Note that the AMPPNP γ-phosphate in NmFic is found disordered <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0064901#pone.0064901-Engel1" target="_blank">[8]</a> and therefore not shown.