The effect of YLT322 on cell morphology and viability of cancer cells.

<p><b>A.</b> Flow cytometric analysis of PI-stained HCC cell lines, including HepG2, SMMC-7721, Bel-7402 and Bel-7404, after treatment with 2 µM YLT322 for 48 hours. <b>B.</b> Statistical results of apoptosis assays presented as surviving cells (percentage of untreated control). Data are expressed as mean ± SD. for at least 3 independent experiments. (*p<0.05; **p<0.01; ***p<0.001). <b>C.</b> Fluorescence microscopy analysis of Hoechst 33342-stained HepG2 cells after incubation with varying concentrations of YLT322 (0 µM (a), 0.5 µM (b), 1 µM (c), 2 µM (d)) for 24 hours (×40). <b>D.</b> Flow cytometric analysis of cells stained with Annexin V-FITC/PI after treatment with various concentrations (0 µM, 0.5 µM, 1 µM, 2 µM) of YLT322 for 12 to 48 hours.