iNKT activity in vivo.

<p>(<b>A</b>) In vivo cytokine secretion after i.p. administration of 2 µg α-GalCer in 200 µl of PBS to healthy, non-terminal and terminal sick 5T33MM mice. Serum was collected during a time course, responses were followed by determining IFN-γ and IL-4 levels by ELISA (mean ± SD is shown from 5 mice in each group). (<b>B</b>) Kaplan-Meier survival assay. Mice were intravenously injected with a single dose of matured DCs (6×10⁵ cell/mouse) loaded with α-GalCer (100 ng/mL) or unloaded at the same day of inoculation with 5T33MM cells (n = 8 mice/group, p = 0.0366). Vehicle mice had an average survival of 22 days, α-GalCer treated mice 29 days.