Development of an <i>in Vitro</i> 3D Tumor Model to Study Therapeutic Efficiency of an Anticancer Drug

The importance and advantages of three-dimensional (3D) cell cultures have been well-recognized. Tumor cells cultured in a 3D culture system as multicellular tumor spheroids (MTS) can bridge the gap between <i>in vitro</i> and <i>in vivo</i> anticancer drug evaluations. An <i>in vitro</i> 3D tumor model capable of providing close predictions of <i>in vivo</i> drug efficacy will enhance our understanding, design, and development of better drug delivery systems. Here, we developed an <i>in vitro</i> 3D tumor model by adapting the hydrogel template strategy to culture uniformly sized spheroids in a hydrogel scaffold containing microwells. The <i>in vitro</i> 3D tumor model was to closely simulate an <i>in vivo</i> solid tumor and its microenvironment for evaluation of anticancer drug delivery systems. MTS cultured in the hydrogel scaffold are used to examine the effect of culture conditions on the drug responses. Free MTS released from the scaffold are transferred to a microfluidic channel to simulate a dynamic <i>in vivo</i> microenvironment. The <i>in vitro</i> 3D tumor model that mimics biologically relevant parameters of <i>in vivo</i> microenvironments such as cell–cell and cell–ECM interactions, and a dynamic environment would be a valuable device to examine efficiency of anticancer drug and targeting specificity. These models have potential to provide <i>in vivo</i> correlated information to improve and optimize drug delivery systems for an effective chemotherapy