The biological activity of wild-type Cdt proteins.

<p><b>A.</b> Effect of wild-type Cdt proteins and CDT holotoxins on the proliferation of CHO cells A. Wild-type recombinant CdtA, B. Wild-type recombinant CdtB, C. Wild-type recombinant CdtC. The amount of each protein, in µg is shown above the image. Data showed one representative experiment of three independent experiments. ^##<i>p</i><0.01 and^###<i>p</i><0.001 versus the experiments. <b>B.</b> Effect of reconstituted wild-type CDTs on the G2M cycle arrest of Hela cells. The cell cycle of Hela cells in each group was measured by Flow cytometry (a–h). The percent cells of G2M cycle (%) was listed in the figures and histograms. Data showed one representative experiment of three independent experiments. ** <i>p</i><0.01 and *** <i>p</i><0.001 versus the control values.^###<i>p</i><0.001 versus the experiments. The amount of each protein, in µg is shown below the image. CdtA 10 µg + CdtB 3 µg + CdtC 10 µg showed the optimal quality ratio in the percent of cells accumulating at G2/M.