TNF-α deficiency did not affect CBDL+CDL-induced liver injury.

<p>TNF-α^+/+ and TNF-α^−/− mice received CBDL+CDL. The animals were sacrificed at the indicated times. (A) The injured lesion in the livers was assessed by H&E staining (original magnification: 40×). (B) Serum ALT and total bilirubin levels were compared at the indicated times. (C) Expression of F4/80 in the livers was examined by immunohistochemistry (original magnification: 200×). F4/80 positive area was compared (right panel). (D) CD3⁺ cells in the livers were examined by immunohistochemistry (original magnification: 200×). Number of CD3⁺ cells was compared. Data are mean ± SD from at least 5 independent experiments. *, P<0.05 vs. sham using a 2-tailed Student’s t-test.