TNF-α deficiency reduced liver fibrosis after CBDL+CDL.

<p>TNF-α^+/+ and TNF-α^−/− mice received CBDL+CDL. The animals were sacrificed on 3 (A) or 21 (B, C) days after the surgery. (A) Serum TNF-α levels were measured by ELISA. (B, C) Collagen deposition was assessed by Sirius red staining (B, original magnification: 40×, graph in right panel) and measurement of hydroxyproline content (C). Data are mean ± SD from at least 5 independent experiments. *, P<0.05 using a 2-tailed Student’s t-test.