TNF-α deficiency did not affect hepatocyte regeneration after CBDL+CDL.

<p>TNF-α^+/+ and TNF-α^−/− mice received CBDL+CDL. The animals were sacrificed 7 days after the surgery. (A) Expression of Ki67 in the livers was examined by immunohistochemistry (original magnification: 40×). Number of Ki67⁺ cells was compared (right panel). Data are mean ± SD from at least 5 independent experiments. *, P<0.05 using a 2-tailed Student’s t-test. (B) The protein extracts from the livers were subjected to SDS-PAGE and immunoblotting was performed with anti-cyclin E and -GAPDH antibodies. The results shown are representative of at least 3 independent experiments.