FK506 reduces expression of type I collagen and αSMA in hepatic fibrosis.

<p>A. RT-PCR analysis of collagen α1(I) mRNA (COL1A1), collagen α2(I) mRNA (COL1A2), αSMA mRNA and actin (ACT) mRNA in total liver RNA from the experimental animals. The results from 4 animals in each group are shown. B. and C. Quantification of expression of collagen α1(I) and α2(I) mRNA after normalization to actin mRNA expression. The data from all 8 animals were used and presented as average and±1 SEM. *** represents significance at p<0.01. D. Western blot analysis of total proteins from the livers of experimental animals. Collagen α1(I) polypeptide (COL α1(I)), αSMA and actin (ACT), as a loading control, from 4 animals of each group is shown. E. Quantification of expression of collagen α1(I) polypeptide after normalization to actin expression. The data from all 8 animals were used and presented as average and±1 SEM. *** represents significance at p<0.01. F. Quantification of αSMA expression after normalization to actin expression.