Type IV pili biogenesis cluster <i>of R. flavefaciens</i> 007C.

<p>The protein Pil3, encoded by the <b><i>pil3</i></b> gene, shares 51% amino acid identity to the cellulose-binding protein (CbpC) of <i>R. albus</i> 8 and 43% amino acid identity to <i>R. albus</i> 20 protein GP25 (a protein that was shown to be underproduced by spontaneous cellulose adhesion-defective mutant D5). The gene downstream (<i>pil4</i>) encodes another protein with prepilin-type IV N-terminal domain, whereas the genes upstream encode: putative PilT−/PilB-like ATPases (<i>pilT, pilB</i>), prepilin peptidase (<i>pilD</i>), two proteins with pseudopilin PulG-like domains (<i>pil1</i>, <i>pil2</i>), type IV pilus assembly protein (<i>pilM</i>) and a type II secretion system protein (<i>pilF</i>). OrfX refers to open reading frame without recognized signal sequences.