Lack of influenza specific T_CD8+ responses in Batf3^o/o mice.

<p>(A) Pre-gating strategy to identify DC (B–C) DC from the inguinal and mediastinal lymph nodes of wildtype or Batf3^o/o mice on a B6 background were analyzed for their expression of either (B) CD8 and CD205 or (C) CD8 and CD103. Representative plots from 3-pooled mice from two independent experiments are shown. (D–E) B6 and Batf3^o/o mice were infected with PR8. On day 10, the absolute number of influenza specific T cells specific for defined peptide sequences were measured in the spleen (D) or BAL (E). The specific T cell response was elucidated following stimulation without peptides (Nil) or the peptides NP_366–374, PA_224–233, PB1-F2_62–70, or PB1_703–711. Shown is the absolute number of IFNγ⁺ CD8⁺ T cells, calculated using the following equation: cell count x%PI⁻ x%CD8⁺ x%IFNγ⁺. Average is taken from between 5–6 mice per group over two independent experiments and the error shows the SEM.