<p>(A) <i>Tc-GSK-3</i> expression decreases after <i>Tc-GSK-3</i> dsRNA injection. (B) Number of laid eggs diminishes 50% after <i>Tc-GSK-3</i> RNAi when compared to the control. (C) Number of hatching larvae decreases after <i>Tc-GSK-3</i> RNAi to about 20% of the control. (D) Glycogen content increases in <i>Tc-GSK-3</i> RNAi eggs when compared to the control (<i>LacZ</i> RNAi eggs). Asterisk indicates that the difference between the two groups is statistically significant (p<0,05).</p
Experimental approach (A). Weight of dsRNA-injected insects following a blood meal. Data are the mea...
<p>Late instar <i>T. castaneum</i> larvae were injected with 0.2 µg of dsRNA for <i>TcCPR27</i> or <...
RNA interference-mediated gene silencing was shown to be an efficient tool for validation of targets...
<p>dsRNA targeting TcMCORP or TcVer was injected into <i>T. castaneum</i> pharate pupae. Injected fe...
<p>(A) Injection of dsRNA for <i>TcKnk2</i> into penultimate instar larvae, last instar larvae and p...
<p> (A) dsRNAs specific for different exons and intron of <i>TcKnk3</i> (exon 6, exon 8, exon 9 and ...
<p>A) The top three images represent ovaries dissected from TcVer knockdown insects (control), and t...
<p>Unfed Wol<sup>+</sup> females were injected with 1 µg of either unrelated double-stranded RNA (β-...
<p>Eggs with 0, 6, 12, 24 and 48 HAO were used to determine the following metabolites. (A) Protein c...
<p>(A) Comparison of the numbers of eggs laid by adult females (n = 20) following dsRNA injection fo...
<p>(A,B) Ovary morphology in (A) control ovaries (injected with LacZ dsRNA) and (B,B’) After <i>Tc-H...
<p>GSK-3 knockdown does not affect adult A. fluviatilis engorgement and development. Unfed Wol+ fema...
<p>(A) Expression of <i>TcRtv</i> during <i>T. castaneum</i> development. Total RNA was extracted at...
<p>(A) Normalized number of <i>dsneo</i> and <i>dszld</i> RNAi collected eggs. <i>zld</i> pRNAi redu...
<p>Unfed Wol+ females were injected with 1 µg of either unrelated double-stranded RNA (β-gal, dashed...
Experimental approach (A). Weight of dsRNA-injected insects following a blood meal. Data are the mea...
<p>Late instar <i>T. castaneum</i> larvae were injected with 0.2 µg of dsRNA for <i>TcCPR27</i> or <...
RNA interference-mediated gene silencing was shown to be an efficient tool for validation of targets...
<p>dsRNA targeting TcMCORP or TcVer was injected into <i>T. castaneum</i> pharate pupae. Injected fe...
<p>(A) Injection of dsRNA for <i>TcKnk2</i> into penultimate instar larvae, last instar larvae and p...
<p> (A) dsRNAs specific for different exons and intron of <i>TcKnk3</i> (exon 6, exon 8, exon 9 and ...
<p>A) The top three images represent ovaries dissected from TcVer knockdown insects (control), and t...
<p>Unfed Wol<sup>+</sup> females were injected with 1 µg of either unrelated double-stranded RNA (β-...
<p>Eggs with 0, 6, 12, 24 and 48 HAO were used to determine the following metabolites. (A) Protein c...
<p>(A) Comparison of the numbers of eggs laid by adult females (n = 20) following dsRNA injection fo...
<p>(A,B) Ovary morphology in (A) control ovaries (injected with LacZ dsRNA) and (B,B’) After <i>Tc-H...
<p>GSK-3 knockdown does not affect adult A. fluviatilis engorgement and development. Unfed Wol+ fema...
<p>(A) Expression of <i>TcRtv</i> during <i>T. castaneum</i> development. Total RNA was extracted at...
<p>(A) Normalized number of <i>dsneo</i> and <i>dszld</i> RNAi collected eggs. <i>zld</i> pRNAi redu...
<p>Unfed Wol+ females were injected with 1 µg of either unrelated double-stranded RNA (β-gal, dashed...
Experimental approach (A). Weight of dsRNA-injected insects following a blood meal. Data are the mea...
<p>Late instar <i>T. castaneum</i> larvae were injected with 0.2 µg of dsRNA for <i>TcCPR27</i> or <...
RNA interference-mediated gene silencing was shown to be an efficient tool for validation of targets...