C/EBPβ structural mutants define distinct myeloid cell trans-differentiation outcomes.

<p>A. Representative FACS plots depicting the expression of myeloid cell markers Ly-6C, M-CSFR, and Ly-6G on 9 days trans-differentiated cells. FACS plots represent GFP⁺ CD11b⁺ cell populations, for MSCV control - GFP⁺ CD19⁺ cells. B. Distribution of the myeloid subpopulations among the reprogrammed GFP⁺ CD11b⁺ cells after staining as in A and presented as mean ± SEM. N - number of repetitions. Gr - neutrophil granulocytes, iM and rM - inflammatory and resident monocytes/macrophages, respectively, DC - dendritic cells. C. Expression of the DC markers CD11c, MHC-II and CD86 on the reprogrammed Ly-6C^– M-CSFR^– cells. Histograms represent GFP⁺ CD11b⁺ Ly-6C^– M-CSFR^– gated cells (color coded as the corresponding population on the Ly-6C/M-CSFR plot in A). “++”, “+”, “med” and “−” represent the expression levels of MHC-II and CD86 antigens. D. Cytospins of control MSCV infected CD19⁺ cells and CD11b⁺ cells reprogrammed by WT C/EBPβ or deletion mutants. B – B cells, M – macrophages, Gr – neutrophil granulocytes, * - monocytes/DCs.