Androgen ablation increases Klf5 expression and the number of Klf5-expressing cells in mouse prostates.

<p>A. Detection of Klf5 by IF staining with total Klf5 antibody in the dorsal prostate at different times after castration (Magnification: 100X, n = 3 for each time point). B. Higher magnification (200X) of cells stained for Klf5 and DAPI in normal prostates and prostates undergoing androgen ablation for 5 weeks. C. Double IF staining of Klf5 and basal cell marker Ck14 in prostates after 5 weeks of castration (Magnification: 400X). D. IHC staining of unAc-Klf5 in intact and androgen-depleted prostates (5 weeks). E. IHC staining of Ac-Klf5 in intact and androgen-depleted prostates (3 and 5 weeks).