Confirmation of <i>Klf5</i> deletion mediated by the expression of Cre in mouse prostates.

<p>A. Breeding strategy for the production of mice with the three desired genotypes of <i>Klf5</i>. B. PCR-based genotyping of <i>Klf5</i>, floxed <i>Klf5</i>, and <i>PB-Cre</i>, with <i>Il-2</i> as a control for the detection of <i>Cre</i>. C. Detection of <i>Klf5</i> knockout in different tissues from 9-week-old heterozygous mice. Primers F and R1 amplify the wildtype allele while primers F and R2 amplify the knockout allele. Tissues in the lanes are: 1, anterior prostate; 2, dorsal prostate; 3, lateral prostate; 4, ventral prostate; 5, seminal vesicle; 6, urethra; 7, testis; 8, bladder; 9, heart; 10, brain; 11, lung; 12, liver; 13, stomach; 14, kidney; 15, spleen; 16, small intestine; 17, colon; 18, salivary gland; and 19, tail. D. Detection of <i>Klf5</i> mRNA by real-time RT-PCR in whole prostates of 13-week-old mice with different deletion status of <i>Klf5</i> (w/w, wildtype; f/w, heterozygous deletion; f/f, homozygous deletion). E. Reduced expression of Klf5 protein by the knockout of <i>Klf5</i>, as detected by IF staining in prostates from 16-week-old mice (Magnification: 200X).