Alignment of Amino Acid Sequences of the Fibre Expressed Cotton PMEs with Other Closely Related Plant PMEs.

<p>All sequences are the full-length sequence where available aligned using ClustalW2 (<a href="http://www.ebi.ac.uk/Tools/clustalw2/index.html" target="_blank">http://www.ebi.ac.uk/Tools/clustalw2/index.html</a>). Identical amino acids are in black, with less conserved residues in shades of grey. The catalytic residues Asp (D) and Arg (N) are indicated by vertical arrows below the sequences. The putative transmembrane domains are boxed. The signal peptide is shown by the horizontal arrows. The predicted cleavage sites for mature protein are indicated by vertical arrows above the sequences. The triangle shows the potential N-linked glycosylation sites. A) <i>G. hirsutum</i> PME1 and 2 aligned with PMEs from <i>Arabidopsis</i> (AtPME2, & AtPME3), orange (CsPME2 & 3), tobacco (PMEU1) and poplar (PttPME1). Note that the <i>GhPME1</i> cDNA is not full-length at the 5-end. B) <i>G. hirsutum</i> PME3 and 4 aligned with PMEs from <i>Arabidopsis</i> (AtPME32) and pear (PcPME4). C) <i>G. hirsutum</i> PME5 aligned with the PME from orange (CsPME2).